| Tube 용량 = Sample Capacity
(i.e. 1mL, 3mL, 12mL, 20mL, 60mL, 150mL)
Solvent와 sample을 한번에 통과활 수 있는 최대 volume
Sorbent 충진물 양 = Analyte Capacity
( i.e. 30mg, 50mg, 60mg, 100mg, 150mg, 200mg, 500mg, 1g, 2g, 5g, 10g, 20g, 50g, 70g)
Sorbent에 머물게될 Analyte의
최대량을 결정
● Silica-based sorbent (5%)
(i.e. 100mg cartridge retain 5mg)
● Polymeric sorbent (1-~15%)
● Ion exchange SPE
analyte와 sorbent의 interation에 따라 다름
Bed Volume
Bed volumne은 SPE method에 필요한 solvent의 양과 관계가 있습니다
Pore Size
SPE에 loading하는 sample의 분자량과 관계가 있습니다 큰 pore size SPE에는 큰 분자량의 sample이 적합합니다
Selecting the Proper Tube
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Sorbent Wash and Elution Volumes
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Sample Pretreatment for SPE |
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만약 sample이 이러한 조건에 맞지 않는다면 SPE 사용 전에
반드시 원활한 extration을 위한 예비조치가 필요합니다
아래 내용은 sample matrix에 따라 일반적인 사전처리(pretreatment)를 요역해 놓은 내용입니다 |
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● Biological samples (liquid)
Dilute sample 1:1 with appropriate buffer, precipitate proteins if proteinaceous (ZnSO4, Acetonitrile), hydrolyze urinary glucuronides, disruption of protein binding (sonication, enzymatic, acids/bases) | |
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● Ointments, creams
Oil based : dissolve in non-polar organic (hexane) and
extract via polar SPE
Water based: dissolve in water or water miscible organic
Methanol and extract via non-polar SPE | |
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● Fruit, vegetable, Herbs
Homogenize with organic or aqueous solvent depending upon analyte solubility and filter supernatant. Use appropriate SPE mechanism for the dissolution solvent (Hexane = polar mechanism, aqueous = non-polar mechanism; Methanol/ Acetonitrile = either non-polar or polar after proper dilution) |
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● Soil, sludge
Homogenize with organic or aqueous solvent depending upon analyte solubility, settle, decant and filter supernatant, perform Soxhlet extraction |
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● Biological samples (solid)
Homogenize with organic or aqueous solvent depending upon analyte solubility, settle, decant, centrifuge or filter supernatant. Perform direct Matrix Solid Phase Dispersion (MSPD) extraction on tissue | | | |
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Proper Tube
